ANTIOXIDANT AND ANTIRADICAL ACTIVITY IN VITRO OF HERB EXTRACTS OF SANGUISORBA OFFICINALIS L., GATHERED IN VARIOUS DEVELOPMENT STAGES
Main Article Content
Abstract
The subject of the investigation. 70 % water-ethanol extracts of Sanguisorba officinalis L. herb.
The purpose of the investigation – a comparative research of antioxidant and antiradical activity of 70 % water-ethanol extracts of Sanguisorba officinalis L. herb, gathered during various development stages – beginning of growing, budding, blooming and fruit-bearing, was performed using in vitro methods.
Methods of investigation. Antioxidant activity was evaluated by inhibiting lipid peroxidation reaction and adrenalin autoxidation. Antiradical activity of the extracts was determined by the ability to inactivate DPPH-, ABTS-radicals and by their ability to bind nitrogen oxide molecules, inhibiting of superoxide, hydroxyl radical and ions Fe+2chelate activity.
Main results. It was determined that herb extracts, gathered during budding and blooming stages, had higher antioxidant and antiradical activity which is explained by high content of polyphenolic compounds, in particular flavonoids in these periods.
Scope. The results can be used for creation of new medicinal products from medicinal plants possess antioxidant action.
Conclusions. The results of these tests allow to consider Sanguisorba officinalis L. herb, gathered during budding and blooming stages, as perspective antioxidant medicinal herb.Keywords
Article Details
Information about financing and conflict of interests
The authors declare that they have no apparent or potential conflicts of interest related to the publication of this article.
This work is licensed under a Creative Commons Attribution 4.0 License.
How to Cite
References
Gupta D. Methods for determination of antioxidant capacity: a review. Intern. J. of Pharmaceutical Sciences and Research. 2015; 6(2): 546-566
Pristom AM, Benhamed M. Oxidative stress and cardiovascular disease. Part 1. Lechebnoe delo: nauchno-prakticheskij terapevticheskij zhurnal. 2012; 1(23): 21-28. Russian (Пристом А.М., Бенхамед М. Оксидативный стресс и сердечно-сосудистые заболевания. Часть 1. //Лечебное дело: научно-практический терапевтический журнал. 2012. № 1(23). С. 21-28)
Men'shchikova EV, Lankin VZ, Kandalintseva NV. Phenolic antioxidants in biology and medicine. Structure, properties, mechanisms of action. LAP, 2012. 495 p. Russian (Меньщикова Е.В., Ланкин В.З., Кандалинцева Н.В. Фенольные антиоксиданты в биологии и медицине. Строение, свойства, механизмы действия. LAP, 2012. 495 с.)
Egorova NО. The polyphenol content in aerial parts of Burnet (Sanguisorba officinalis L.). Materials of all-Russian scientific conference of students and postgraduates with international participation «Young pharmacy – potential future», 20-21 April 2011, Saint Petersburg: «Publishing house SPCPA». 2011. P. 42-44. Russian (Егорова Н.О. Содержание полифенолов в надземной части кровохлебки лекарственной (Sanguisorba officinalis L.) //Матер. Всерос. науч. конф. студ. и аспирантов с междунар. участием «Молодая фармация – потенциал будущего», 20-21 апреля 2011 г. СПб.: «Изд-во СПХФА». 2011. С. 42-44)
Maltseva EM, Egorova NO, Egorova IN. Quantitative determination of total content of flavonoids in the grass of Burnet. Bulletin of the Ural medical academic science. 2011; 3(1): 68. Russian (Мальцева Е.М., Егорова Н.О., Егорова И.Н. Количественное определение суммарного содержания флавоноидов в траве кровохлебки лекарственной //Вестник уральской медицинской академической науки. 2011. № 3(1). С. 68)
FFS 1.5.3.0008.15 the Determination of tannins in herbal drugs and medicinal plant preparations. Available at: http://193.232.7.120/feml/clinical_ref/pharmacopoeia_2/HTML/#417/z (accessed 25.12.2016). Russian (ОФС 1.5.3.0008.15 Определение содержания дубильных веществ в лекарственном растительном сырье и лекарственных растительных препаратах. Доступ: http://193.232.7.120/feml/clinical_ref/pharmacopoeia_2/HTML/#417/z (Дата обращения 25.12.2016)
Mukhamadiyarov RA, Radionov IA, Razumov AS, Bogdanov MV. Efficiency of liposomal forms of antioxidants for correcting oxidative stress in chronic pancreatitis in experiment. Nanotechnol. Russia. 2015; 10(1): 135-139
Madhu CS, Manukumar HM, Puttalingaiah B. New-vista in finding antioxidant and anti-inflammatory property of crude protein extract from Sauropus androgynus leaf. Acta Sci. Pol., Technol. Aliment. 2014; 13(4): 375-383
Rahini D, Anuradha R. In-vitro antioxidant activity of Artabotrys hexapetallus. Research Journal of Pharmaceutical, Biological and Chemical Sciences. 2014; 5(2): 396-405
Adesanwo JK, Makinde OO, Obafemi CA. Phytochemical analysis and antioxidant activity of methanol extract and betulinic acid isolated from the roots of Tetracera potatoria. J. of Pharmacy Research. 2013; 6: 903-907
Pham Thi Be Tu, Shinkichi Tawata. Anti-Oxidant, Anti-Aging and Anti-Melanogenic Properties of the Essential Oils from Two Varieties of Alpinia zerumbet. Molecules. 2015; 20: 16723-16740
Patel DS, Shah PB, Managoli NB. Evaluation of in-vitro Anti-oxidant and Free Radical Scavenging activities of Withania somnifera and Aloe vera. Asian J. Pharm. Tech. 2012; 2(4): 143-147
Ryabinina EI, Zotova EE, Vetrova EN, Ponomareva NI, Ilyushina TN. A new approach in the evaluation of antioxidant activity of plant materials in the study of the process of auto-oxidation of adrenaline. Chemistry of vegetable raw materials. 2011; 3: 117-121. Russian (Рябинина Е.И., Зотова Е.Е., Ветрова Е.Н., Пономарева Н.И., Илюшина Т.Н. Новый подход в оценке антиоксидантной активности растительного сырья при исследовании процесса аутоокисления адреналина / Е. И. Рябинина [и др.] //Химия растительного сырья. 2011. №3. С. 117-121)
Toropova AA, Nikolaev SM, Verlan NV. Antioxidant activity of complex herbal remedy «Pancreatic» and its components in model systems. Siberian medical journal (Irkutsk). 2014; 7: 119-121. Russian (Торопова А.А., Николаев С.М., Верлан Н.В. Антиоксидантная активность комплексного растительного средства «Панкреофит» и его компонентов в модельных системах //Сибирский медицинский журнал (Иркутск). 2014. № 7. С. 119-121)