ASSOCIATION BETWEEN TNF-Α-308 G/A GENE POLYMORPHISM AND GASTRIC CANCER RISK
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Abstract
Subject of research. Venous blood of patients with gastric cancer hospitalized with gastrointestinal bleeding. The study group included 14 men (45 %), 17 women (55 %), average age 63.2 ± 3.5 (M ± m). The comparison group included 58 patients undergoing medical examination prior to surgical treatment (replacement arthroplasty). Gender distribution of the patients in the comparison group – 19 men (33 %), 39 women (67 %), average age 62.4 ± 1.8 (M ± m)
Objective – to determine the risk of development of gastric cancer based on the analysis of polymorphism of tumor necrosis factor alpha.
Research methods. DNA isolation was performed using phenol-chloroform extraction. Genotyping of single nucleotide substitutions in the TNF-α gene at -308 (G; A) was performed using real-time PCR with competitive TaqMan probes complementary to polymorphic DNA regions. The accuracy of the genotyping is confirmed by sequencing.
Key findings. The distribution of TNF-α genotypes at -308 (G; A): GG 58 %, GA 42 % and AA 0 % in patients with gastric cancer and GG 79 %, GA 21 % and AA 0 % in the comparison group. In the group of patients with cancer the heterozygous GA genotype of the TNF-a gene was more frequent than in the comparison group: 42 % versus 21 % (χ2 = 4.514; р = 0,034). Calculation of odds ratio has shown that the GA genotype is associated with risk of gastric cancer development (OR = 2.769; lower limit 95% CI = 1.065, upper limit 95% CI = 7.197).
Scope of application. Formation of gastric cancer development risk groups.
Conclusions. The results of our study demonstrate the association between the heterozygous GA TNF-a-308 genotype and the risk of development of gastric cancer.
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Statua of cancer the population of Russia in 2017 /ed. Caprina AD, Starinsky VV, Petrova GV. M.: P.A. Hertsen Moscow Oncology Research Center. 2018. 236 p. Russian (Состояние онкологической помощи населению России в 2017 году /под ред. Каприна А.Д, Старинского В.В., Петровой Г.В. М.: МНИОИ им. П.А. Герцена. 2018. 236 с.)
Bausys R, Bausys A, Vysniauskaite I, Maneikis K, Stratilatovas E, Strupas K. Surgical treatment outcomes of patients with T1-T2 gastric cancer: does the age matter when excellent treatment results are expected? World J of Surgical Oncology. 2018; 16(1): 40. DOI: 10.1186/s12957-018-1388-4
Fujiwara Y, Fukuda S, Tsujie M, Ishikawa H, Kitani K, Inoue K, et al. Effects of age on survival and morbidity in gastric cancer patients undergoing gastrectomy. World J Gastrointest Oncol. 201; 9(6): 257-262. DOI: 10.4251/wjgo.v9.i6.257
Ben Kridis W, Marrekchi G, Mzali R, Daoud J, Khanfir A. Prognostic factors in metastatic gastric carcinoma. Experimental Oncology. 2019; 41(2): 173-175. DOI: 10.32471/exp-oncology.2312-8852.vol-41-no-2.13283
Zemlyanoy VP, Sigua BV, Gubkov II, Zakharov EA, Mavidi IP, Mikhailov GA, Sakhno DS. Diagnosis and treatment of gastric cancer complicated by bleeding. Herald of North-Western State Medical University named after II Mechnikov. 2017; 9(1): 15-20. Russian (Земляной В.П., Сигуа Б.В., Губков И.И., Захаров Е.А., Мавиди И.П., Михайлов Г.А., Сахно Д.С. Диагностика и лечение рака желудка, осложненного кровотечением //Вестник СЗГМУ им. И.И. Мечникова. 2017. Т. 9, № 1. С. 15-20)
Kim Y-I, Choi J. Endoscopic Management of Tumor Bleeding from Inoperable Gastric Cancer. Clinical Endoscopy. 2015; 48(2): 121-127. DOI: 10.5946/ce.2015.48.2.121
Zheng W, Zhang S, Zhang S, Min L, Wang Y, Xie J, et al. The relationship between tumor necrosis factor‑α polymorphisms and gastric cancer risk: An updated meta‑analysis. Biomedical Reports. 2017; 7(2): 133-142. DOI: 10.3892/br.2017.934
Novikov DG, Kononov AV, Pomorgailo EG, Mozgovoy SI. Assessment of the risk of development of intestinal-type gastric cancer in pactients with cytokine gene polymorphisms. Medicine in Kuzbass. 2010; 4: 21-24. Russian (Новиков Д.Г., Кононов А.В., Поморгайло Е.Г., Мозговой С.И. Оценка риска развития рака желудка кишечного типа у носителей полиморфных аллелей генов цитокинов //Медицина в Кузбассе. 2010. № 4. С. 21-24)
Yang JP, Hyun MH, Yoon JM, Park MJ, Kim D, Park S. Association between TNF-α-308 G/A gene polymorphism and gastric cancer risk: a systematic review and meta-analysis. Cytokine. 2014; 70(2): 104-114. DOI: 10.1016/j.cyto.2014.07.005